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Registro Completo |
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Biblioteca(s): |
Embrapa Amapá. |
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Data corrente: |
29/11/2022 |
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Data da última atualização: |
29/11/2022 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
CAVALCANTE, M. de A.; OLIVEIRA, J. dos S.; BARRETO, M. S. da S.; PINHEIRO, L. P.; CANTUÁRIA, P. de C.; BORGES, W. L.; SILVA, G. A. da; SOUZA, T. M. de. |
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Afiliação: |
MARÍLIA DE A. CAVALCANTE, INSTITUTO FEDERAL DO AMAPÁ; JANYNA DOS S. OLIVEIRA, INSTITUTO FEDERAL DO AMAPÁ; MAYRA S. DA S. BARRETO, UNIVERSIDADE DO ESTADO DO AMAPÁ; LUCAS P. PINHEIRO, UNIVERSIDADE DO ESTADO DO AMAPÁ; PATRICK DE C. CANTUÁRIA, INSTITUTO DE PESQUISAS CIENTÍFICAS E TECNOLÓGICAS DO ESTADO DO AMAPÁ; WARDSSON LUSTRINO BORGES, CPAF-AP; GABRIEL A. DA SILVA, UNIVERSIDADE DO ESTADO DO AMAPÁ; TIAGO MARCOLINO DE SOUZA, UNIVERSIDADE DO ESTADO DO AMAPÁ. |
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Título: |
An HPLC method to determine phenolic compounds of plant extracts: application to Byrsonima crassifolia and Senna alata leaves. |
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Ano de publicação: |
2022 |
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Fonte/Imprenta: |
Pharmacognosy Research, v. 14, n. 4, p. 395-404, 2022. |
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DOI: |
10.5530/pres.14.4.58 |
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Idioma: |
Inglês |
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Conteúdo: |
Background: The Amazonian Region has a variety of medicinal plants with bioactive compounds, whose characterization could present the potential for sustainable development. Objectives: A method for separating, identifying, and quantifying a mixture of nine phenolic compounds (gallic acid, 3-hydroxybenzoic acid, p-coumaric acid, catechin, myricetin, rutin, quercetin, kaempferol, and cyanidin) was developed, validated, and applied to analyze aqueous and hydroethanolic extracts from Byrsonima crassifolia (L.) Kunth and Senna alata (L.) leaves. Materials and Methods: The separation was carried out by HPLC, using a Shim-pack VP-ODS C18 column (5 μm, 150 x 4.6 mm) at 40°C. Detection was performed at 254 nm and separation occurred in 35 min. Results: The optimized method was validated for each of the nine phenolic compounds. The calibration curve for the phenolic compound standards showed suitable linear fitting and exhibited correlation coefficients greater than 0.990. The LOD and LOQ varied between 6.2807 - 14.8851 μg mL-1 and 6.8002 - 16.0071 μg mL-1, respectively. The method was found to be robust for changes of ±2 ml in mobile phase composition. Byrsonima crassifolia aqueous extracts indicated contents of gallic acid, catechin, rutin, and cyanidin whereas hydroethanolic one did not show the first substance. Senna alata aqueous extract presented only 3-hydroxybenzoic acid and rutin whereas myricetin, cyanidin, quercetin, and kaempferol were also identified in the hydroethanolic one. Conclusion: The HPLC method is efficient, precise, accurate, and sensitive to determining phenolic compounds in plant extracts and it is recommended for efficient assays in routine work. MenosBackground: The Amazonian Region has a variety of medicinal plants with bioactive compounds, whose characterization could present the potential for sustainable development. Objectives: A method for separating, identifying, and quantifying a mixture of nine phenolic compounds (gallic acid, 3-hydroxybenzoic acid, p-coumaric acid, catechin, myricetin, rutin, quercetin, kaempferol, and cyanidin) was developed, validated, and applied to analyze aqueous and hydroethanolic extracts from Byrsonima crassifolia (L.) Kunth and Senna alata (L.) leaves. Materials and Methods: The separation was carried out by HPLC, using a Shim-pack VP-ODS C18 column (5 μm, 150 x 4.6 mm) at 40°C. Detection was performed at 254 nm and separation occurred in 35 min. Results: The optimized method was validated for each of the nine phenolic compounds. The calibration curve for the phenolic compound standards showed suitable linear fitting and exhibited correlation coefficients greater than 0.990. The LOD and LOQ varied between 6.2807 - 14.8851 μg mL-1 and 6.8002 - 16.0071 μg mL-1, respectively. The method was found to be robust for changes of ±2 ml in mobile phase composition. Byrsonima crassifolia aqueous extracts indicated contents of gallic acid, catechin, rutin, and cyanidin whereas hydroethanolic one did not show the first substance. Senna alata aqueous extract presented only 3-hydroxybenzoic acid and rutin whereas myricetin, cyanidin, quercetin, and kaempferol were also identified in the... Mostrar Tudo |
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Palavras-Chave: |
Compostos bioativos; Método cromatográfico. |
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Thesagro: |
Cromatografia; Extrato Vegetal. |
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Thesaurus Nal: |
Bioactive compounds; Plant extracts. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1148941/1/CPAF-AP-AnHPLCMethod.pdf
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Marc: |
LEADER 02625naa a2200289 a 4500
001 2148941
005 2022-11-29
008 2022 bl uuuu u00u1 u #d
024 7 $a10.5530/pres.14.4.58$2DOI
100 1 $aCAVALCANTE, M. de A.
245 $aAn HPLC method to determine phenolic compounds of plant extracts$bapplication to Byrsonima crassifolia and Senna alata leaves.$h[electronic resource]
260 $c2022
520 $aBackground: The Amazonian Region has a variety of medicinal plants with bioactive compounds, whose characterization could present the potential for sustainable development. Objectives: A method for separating, identifying, and quantifying a mixture of nine phenolic compounds (gallic acid, 3-hydroxybenzoic acid, p-coumaric acid, catechin, myricetin, rutin, quercetin, kaempferol, and cyanidin) was developed, validated, and applied to analyze aqueous and hydroethanolic extracts from Byrsonima crassifolia (L.) Kunth and Senna alata (L.) leaves. Materials and Methods: The separation was carried out by HPLC, using a Shim-pack VP-ODS C18 column (5 μm, 150 x 4.6 mm) at 40°C. Detection was performed at 254 nm and separation occurred in 35 min. Results: The optimized method was validated for each of the nine phenolic compounds. The calibration curve for the phenolic compound standards showed suitable linear fitting and exhibited correlation coefficients greater than 0.990. The LOD and LOQ varied between 6.2807 - 14.8851 μg mL-1 and 6.8002 - 16.0071 μg mL-1, respectively. The method was found to be robust for changes of ±2 ml in mobile phase composition. Byrsonima crassifolia aqueous extracts indicated contents of gallic acid, catechin, rutin, and cyanidin whereas hydroethanolic one did not show the first substance. Senna alata aqueous extract presented only 3-hydroxybenzoic acid and rutin whereas myricetin, cyanidin, quercetin, and kaempferol were also identified in the hydroethanolic one. Conclusion: The HPLC method is efficient, precise, accurate, and sensitive to determining phenolic compounds in plant extracts and it is recommended for efficient assays in routine work.
650 $aBioactive compounds
650 $aPlant extracts
650 $aCromatografia
650 $aExtrato Vegetal
653 $aCompostos bioativos
653 $aMétodo cromatográfico
700 1 $aOLIVEIRA, J. dos S.
700 1 $aBARRETO, M. S. da S.
700 1 $aPINHEIRO, L. P.
700 1 $aCANTUÁRIA, P. de C.
700 1 $aBORGES, W. L.
700 1 $aSILVA, G. A. da
700 1 $aSOUZA, T. M. de
773 $tPharmacognosy Research$gv. 14, n. 4, p. 395-404, 2022.
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Registro original: |
Embrapa Amapá (CPAF-AP) |
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Registro Completo
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Biblioteca(s): |
Embrapa Agropecuária Oeste. |
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Data corrente: |
30/03/2020 |
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Data da última atualização: |
30/03/2020 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
ABREU, H. K. A. de; FACHINELLI, R.; MAGALHÃES, L. da S.; CECCON, G. |
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Afiliação: |
HADASSA KATHYUCI ANTUNES DE ABREU, DOUTORANDA EM AGRONOMIA - UNIVERSIDADE FEDERAL DA GRANDE DOURADOS, DOURADOS, MS; RICARDO FACHINELLI, DOUTORANDO EM AGRONOMIA - UNIVERSIDADE FEDERAL DA GRANDE DOURADOS, DOURADOS, MS; LARISSA DA SILVA MAGALHÃES, GRADUANDA EM AGRONOMIA - CENTRO UNIVERSITÁRIO DA GRANDE DOURADOS, DOURADOS, MS; GESSI CECCON, CPAO. |
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Título: |
Co-inoculação de Bradyrhizobium com Azospirillum sobre a produtividade da soja em solos com e sem palha de braquiária. |
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Ano de publicação: |
2019 |
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Fonte/Imprenta: |
In: JORNADA DE INICIAÇÃO À PESQUISA DA EMBRAPA, 2019, Dourados. Resumos... Brasília, DF: Embrapa, 2019. JIPE 2019 |
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Idioma: |
Português |
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Palavras-Chave: |
Fixação biológica de nitrogênio. |
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Thesagro: |
Cobertura do Solo. |
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Categoria do assunto: |
A Sistemas de Cultivo |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/212059/1/ORAL28.pdf
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Marc: |
LEADER 00639nam a2200157 a 4500
001 2121435
005 2020-03-30
008 2019 bl uuuu u00u1 u #d
100 1 $aABREU, H. K. A. de
245 $aCo-inoculação de Bradyrhizobium com Azospirillum sobre a produtividade da soja em solos com e sem palha de braquiária.$h[electronic resource]
260 $aIn: JORNADA DE INICIAÇÃO À PESQUISA DA EMBRAPA, 2019, Dourados. Resumos... Brasília, DF: Embrapa, 2019. JIPE 2019$c2019
650 $aCobertura do Solo
653 $aFixação biológica de nitrogênio
700 1 $aFACHINELLI, R.
700 1 $aMAGALHÃES, L. da S.
700 1 $aCECCON, G.
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Embrapa Agropecuária Oeste (CPAO) |
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